he same lipid that helps algae swim toward the light also appears to enable one type of brain cell to keep cerebrospinal fluid moving, researchers report. The lipid is ceramide, long known to help keep skin smooth, and now Medical College of Georgia researchers have found ceramide helps make and keep in motion motile cilia found in algae and in brains.
“It’s important to know how you regulate your cilia because they can become dysfunctional by stroke, by Alzheimer’s, by inflammation, even by aging,” said Dr. Erhard Bieberich, neuroscientist in the MCG Department of Neuroscience and Regenerative Medicine, and corresponding author of the study in the journal Molecular Biology of the Cell. “If ceramide is one of the regulators, it may also be a drug target to help normalize the function of cilia.”
In the case of algae, motile cilia work like arms on a swimmer, to help algae reach sunlight, which they use to make sugar for energy and survival, Dr. Bieberich said. “They are fast, always moving,” he said of motile cilia that can make 40-50 whip-like motions per second and are continuously being resorbed and replaced. The MCG researchers were the first to show that ceramide is present in these fast-moving algae cilia where it has likely been at work for hundreds of millions of years.
Algae seemed a good first model to figure out what regulates cilia. Cilia in algae are comparatively big – about 10 times those in mammalian cells – so with just a regular microscope, Dr. Bieberich’s team could easily watch cilia movement. When they applied even a miniscule amount of a fungus toxin known to inhibit ceramide production, the usually agile algae stopped, the cilia shrank and some fell off.
These motile cilia also inhabit a type of support brain cell called ependymal cells. The cells line the cavities of the brain containing cerebrospinal fluid, which nourishes and cleanses the brain. While the researchers have not yet visualized cilia movement in those brain cells, they have found ceramide present in the cilia along with glycogen synthase kinase-3, or GSK3, an enzyme that appears to partner with ceramide to regulate cilia.
As the researchers take baby steps through this ornate process, they now also know that ceramide activates GSK3 but they must still find GSK3’s targets. They do know that in both algae and ependymal cells, the two can be found together. And, without their teamwork in the brain, for example, hydrocephalus – excess fluid on the brain – can result.
Georgia Regents University graduate student Ji Na Kong is the study’s first author. The work was funded by the National Science Foundation and the National Institutes of Health. Next steps including working with Dr. Karl F. Lechtreck, assistant professor of cellular biology at the University of Georgia and a cilia expert, to visualize cilia movement in ependymal cells.